Membrane studies with polarity-dependant and excimer-forming fluorescent probes
نویسندگان
چکیده
منابع مشابه
Membrane studies with polarity-dependent and excimer-forming fluorescent probes.
1. The interaction of electron-transporting particles from heavy mitochondria of ox heart with several fluorescent probes was examined. 2. 1-Anilinonaphthalene-8-sulphonate and 2-(N-methylanilino)naphthalene-6-sulphonate both show an energy-dependent response. 3. Energy transfer between the electron-transporting particles and the dyes and the kinetics of the dye-particle interaction were studie...
متن کاملMembrane Fluorescent Probes: Insights and Perspectives
2-AS: 2-(9-anthroyloxy)stearic acid 12-AS: 12-(9-anthroyloxy)stearic acid 25-NBD-cholesterol: 25-[N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-methyl]amino]27norcholesterol DOPC: dioleoyl-sn-glycero-3-phosphocholine MβCD: methyl-β-cyclodextrin NBD: 7-nitrobenz-2-oxa-1,3-diazol-4-yl NBD-PE: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dipalmitoyl-sn-glycero-3-pho sphoethanolamine REES: red edge excitatio...
متن کاملFluorescent Flippers for Mechanosensitive Membrane Probes
In this report, "fluorescent flippers" are introduced to create planarizable push-pull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biology. Twisted push-pull scaffolds with large and bright dithienothiophenes and their S,S-dioxides as the first "fluorescent flippers" are shown to report on the lateral organization of lipid bilayers with quantum yield...
متن کاملAnalysis of protein folding using polarity-sensitive fluorescent probes
Polarity-sensitive fluorescent probes like 8-anilino-1naphthalenesulphonate (ANS) and 1,1′-bis(4-anilino5-naphthalenesulphonic acid (bis-ANS) have been frequently used to detect equilibrium folding intermediates like the molten globule state, as the formation of the latter involves increase in hydrophobic exposure. The ability of these fluorescent probes to bind to the intermediate state thus p...
متن کاملQM/MM studies of contemporary and novel membrane raft fluorescent probes.
We have studied a number of contemporary and novel membrane probes, selected for their structural similarity to membrane raft components, in order to properly anchor themselves within a sphingolipid/cholesterol rich region. A QM/MM approach was adopted in order to understand the structural and electrostatic influences of fluorescence emission shifts of the probes in different lipid and solvatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1160721